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. 2022 Feb 8;38(6):110347. doi: 10.1016/j.celrep.2022.110347

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

iLTP-inducing protocol promotes LTD at excitatory synapses

(A) Top: Experimental configuration of paired-patch recordings of two connected excitatory hippocampal neurons. LFS was delivered to the postsynaptic neuron. Bottom: Representative averaged traces of excitatory postsynaptic response (EPSCs) before and 30 min after the LFS, as depicted above.

(B) Depression of EPSC amplitude (LTD) after LFS (arrow) (n = 5 neurons, F_38,726 = 4.2, p < 0.001, one-way ANOVA followed by Tukey's test).

(C) Top: Experimental configuration of an excitatory neuron receiving the LFS. The target symbol indicates MNI-glutamate uncaging at an individual spine identified by Homer1c-GFP (inset; scale bar, 1 μm). Bottom: Representative averaged traces of uncaging EPSCs (uEPSCs) before (baseline) and 30 min after LFS (LTD), as indicated above.

(D) Persistent reduction of uEPSC amplitude after LFS (n = 16 synapses from 11 neurons, F_4,68 = 4.7, p < 0.01; one-way ANOVA followed by Tukey's test). Values are expressed as mean ± SEM. ^∗∗p < 0.01, ^∗∗∗p < 0.001.