REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-vGAT-Oyster 550	Synaptic System	131103C3, RRID:AB_887867
Rat monoclonal anti-HA	Roche	1186742300, RRID:AB_10094468
Qdot® 655 F(ab')2-Goat anti-Rat IgG (H + L)	Thermo Fisher	Q-11621MP, RRID:AB_2556477
Bacterial and virus strains
AAV1/2-hSyn-Teal-gephyrin-WPRE	This paper	N/A
Chemicals, peptides, and recombinant proteins
BAPTA	Sigma	85233-19-8
L-NAME	Sigma	N5751
Nifedipine	Sigma	N-7634
Bicuculline	Sigma	14343
MDL28170	Sigma	M-6690
KN-93	Millipore Merck	422708
KN-92	Millipore Merck	422709
APV	Tocris	0105/50
CNQX	Tocris	1045/10
ω-conotoxin MVIIC	Tocris	1084/100U
ω-conotoxin GVIA	Tocris	1085/250U
DPNI-caged-GABA	Tocris	2991/10
MNI-caged-L-glutamate	Tocris	1490/10
Rhod-2 tripotassium salt	AAT Bioquest	21067
Alexa Fluor 647 Hydrazide	Thermo Fisher	A20502
Fast Green FCF	Sigma-Aldrich	F7252
Critical commercial assays
Effectene Transfection Reagent	Qiagen	301427
Experimental models: Organisms/strains
Ai9 (B6.Cg-Gt(ROSA)26Sortm9(CAGtdTomato) Hze/J	Jackson Laboratory, USA	JAX:007909, RRID:IMSR_JAX:007909
PVCRE (B6; 129P2-Pvalbtm1(cre) Arbr/J	Jackson Laboratory, USA	JAX:017320, RRID:IMSR_JAX:017320
Parvalbumin-tdTomato (PV-tdTomato)	This paper	N/A
Recombinant DNA
pEGFP-N1	Clontech	Cat# 632162
pcDNA3 Homer1c::DsRed	Petrini et al., 2009	N/A
pcDNA3 Homer1c::GFP	Petrini et al., 2009	N/A
FingR-Gephyrin-GFP	Gross et al., 2013	N/A
EGFP-Gephyrin	Zita et al., 2007	N/A
Hemagglutinin (HA)-tagged α1 GABAA receptor	Petrini et al., 2011	N/A
pAAV1/2-hSyn-Teal-gephyrin-WPRE	This paper	N/A
Software and algorithms
Metamorph 7.8	Molecular Devices	http://www.moleculardevices.com/Products/Software/Meta-Imaging-Series/MetaMorph.html RRID: SCR_002368
Clampex 10.6	Molecular Devices	http://www.moleculardevices.com/products/software/pclamp.html RRID:SCR_011323
Clampfit 10.7	Molecular Devices	http://www.moleculardevices.com/products/software/pclamp.html RRID:SCR_011323
MATLAB	Mathworks	http://www.mathworks.com/products/matlab/RRID:SCR_001622
GraphPad Prism 6	GraphPad	http://www.graphpad.com/RRID: SCR_002798
Custom program written for MATLAB to reconnect QD trajectories	Petrini et al., 2014; from D Choquet and L Cognet	N/A
Custom program for SPT quantifications	Petrini et al., 2014; from D Choquet and A Serge	N/A
Leica Application Suite X	Leica Microsystems	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/