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. 2021 Dec 22;9(5):2103812. doi: 10.1002/advs.202103812

Figure 3.

Figure 3

In vitro cellular uptake, target ability, and endosomal escape of B1L@SpAcDex‐ATMO‐21 NPs (red fluorescence) measured by CLSM and flow cytometry. a) CLSM images of cell uptake within U87MG cells incubated with naked ATMO‐21, PEI@ATMO‐21 NCs, liposome@ATMO‐21 NPs, SpAcDex‐ATMO‐21 NPs, and B1L@SpAcDex‐ATMO‐21 NPs. Scale bar, 20 µm b) Flow cytometry analysis of the delivery efficiency of B1L@SpAcDex‐ATMO‐21 NPs. c) Analysis of the targeting ability of B1L‐modified NPs. d) Mean fluorescence intensities of SpAcDex‐ATMO‐21 NP‐ and B1L@SpAcDex‐ATMO‐21 NP‐treated U87MG cells after 10 h of incubation. e) CLSM images of endosomal escape of as‐fabricated NPs (naked ATMO‐21, PEI@ATMO‐21 NCs, Liposome@ATMO‐21 NPs, SpAcDex‐ATMO‐21 NPs, and B1L@SpAcDex‐ATMO‐21 NPs) measured by CLSM. Scale bar, 10 µm. f) pH‐responsive endo/lysosomal escape of B1L@SpAcDex‐ATMO‐21 NPs. The corresponding colocalization fluorescence intensity and the colocalization ratios between NPs (red fluorescence) and endososomes (green fluorescence) in U87MG cells when incubated with B1L@SpAcDex‐ATMO‐21 NPs for 10 h measured by CLSM. Scale bar, 10 µm.