Figure 2.

Daily intravenous NR injections lead to sustained NAD⁺ increase in peripheral tissues but not in the liver

Various tissues were analyzed for NAD⁺ and NADH content after four weeks of daily injections of either saline or nicotinamide riboside at a dosage of 100 mg/kg bodyweight. Tissues were harvested between 16 and 20 h after the last injection.

(A) Liver NAD⁺ levels.

(B) Liver NADH levels.

(C) Liver NAD⁺/NADH-ratio.

(D) Quadriceps muscle NAD⁺ levels.

(E) Quadriceps muscle NADH levels.

(F) Quadriceps muscle NAD⁺/NADH-ratio.

(G) Tibialis anterior NAD⁺ levels.

(H) Inguinal white adipose tissue (iWAT) NAD⁺ levels.

All data were normalized to the protein content of the sample and are presented as mean with overlain data points. Two-way ANOVA was performed for all figures, and Tukey’s test was employed to explore the interaction in (D) Statistical significance was indicated in the following manner: Effects of intravenous NR: p < 0.05 = †, p < 0.01 = ††, p < 0.001 = †††. Effects of VWR: p < 0.01 = §§. Interactions are written out in the figures and noted with: p < 0.001 = ∗∗∗. In cases without statistical interactions, the above symbols indicate main effects, while in cases with statistical interactions, the symbols indicate simple effects compared to the appropriate control group. See also Figure S1.