REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse/IgG2b anti-GAPDH	Proteintech	60004-1-Ig
Rat anti-Keap1, clone 144	Sigma-Aldrich	MABS514
Rabbit monoclonal anti-Nrf2	Cell Signaling Technology	12721
Rabbit monoclonal anti-Nqo1	Cell Signaling Technology	62262
Horseradish-peroxidase conjugated goat anti-rabbit secondary antibody	Cell Signaling Technology	7074
Polyclonal rabbit anti-TOM20 antibody	Proteintech	11802-1-AP
Goat anti-rabbit Alexa 569 antibody	Invitrogen	A11036
IRDye® 680RD Donkey anti-Mouse IgG Secondary Antibody	LI-COR	926–68072
IRDye® 680RD Goat anti-Rat IgG Secondary Antibody	LI-COR	926–68076
IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody	LI-COR	926–32211
Chemicals, peptides, and recombinant proteins
Lipopolysaccharide (LPS) from Escherichia coli O111:B4	Sigma	L2630
Polyinosinic–polycytidylic acid sodium salt (Poly(I:C))	Sigma	P0913
2′3′-cGAMP	Invivogen	tlrl-nacga23-1
2′3′-cGAMP Control (Negative, 2′5′GpAp)	Invivogen	tlrl-nagpap
Lipofectamine™ RNAiMAX	Invitrogen	13778075
Lipofectamine™ 2000	Invitrogen	11668019
ON-TARGETplus human NFE2L2 siRNA-SMARTpool	Dharmacon	L-003755-00–0005
siRNA negative control	Dharmacon	D-001810-10-50
4-octyl itaconate (4-OI)	Richard Hartley	Mills et al., (2018)
TBE-31	Tadashi Honda	Saito et al., 2013
2-cyano-3,10-dioxooleana-1,9(11)-dien-28-oic acid (CDDO)	Tadashi Honda	Honda et al., (1998)
R,S-Sulforaphane	LKT Labs	S8044
Phorbol 12-myristate 13-acetate	Sigma	P1585
Omniscript RT Kit	Qiagen	205113
PrimeScript™ RT Master Mix	Takara	RR036A
TaqMan™ Fast Advanced Master Mix	Applied Biosystems	4444557
Fast SYBR® Green Master Mix	Applied Biosystems	4385610
Critical commercial assays
Mouse IFN beta ELISA Kit	Abcam	ab252363
Mouse IL-6 DuoSet ELISA	R&D systems	DY406
Mouse TNF-alpha DuoSet ELISA	R&D systems	DY410
TMTXplex™ Isobaric Mass Tagging Kit	Thermo Fisher Scientific	90406
RNeasy Mini Kit (50)	Qiagen	74104
High-Capacity cDNA Reverse Transcription Kit	Applied Biosystems	4368814
Deposited data
MaxQuant proteome quantification output - TMT	PRIDE	PXD027737
Data-independent acquisition (DIA) proteomics	PRIDE	PXD030455
Differential expression results	This paper	Tables S1, S2, S3, S4, S5, S6 and S7
Experimental models: Cell lines
Murine RAW 264.7 macrophage-like monocytes	ATCC	TIB-71
Human THP1 monocytes	Public Health England/ECACC	88081201
Experimental models: Organisms/strains
Mus musculus C57BL/6 Wild type	In-house	Jackson Laboratory
Mus musculus C57BL/6 Nrf2−/−: Keap1+/+ (Nrf2-KO)	In-house	Higgins et al., (2009)
Mus musculus C57BL/6 Nrf2−/−: Keap1fl/fl (Keap1-KD)	In-house	Taguchi et al., (2010)
Oligonucleotides
Primers, see qPCR primers section in methods	This paper	M/A
Software and algorithms
Metaboanalyst 5.0	Pang et al. (2021)	https://www.metaboanalyst.ca/
Bioconductor package limma via LFQ-analyst	Shah et al. (2020)	https://bioinformatics.erc.monash.edu/apps/LFQ-Analyst/
Enrichr	Kuleshov et al. (2016)	https://maayanlab.cloud/Enrichr/
Bioconductor package clusterProfiler 4.0	Wu et al., 2021	https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
Graphpad Prism 9.2.0	GraphPad	https://www.graphpad.com/scientific-software/prism/
Taqman™ Gene expression assays and qPCR primers
Nqo1 (mouse)	Applied Biosystems	Mm01253561_m1
Cxcl10 (mouse)	Applied Biosystems	Mm00445235_m1
Ifnb1 (mouse)	Applied Biosystems	Mm00439552_s1
Ifit2 (mouse)	Applied Biosystems	Mm00492606_m1
NQO1 (human)	Applied Biosystems	Hs02512143_s1
CXCL10 (human)	Applied Biosystems	Hs00171042_m1
IFNB1 (human)	Applied Biosystems	Hs01077958_s1
NFE2L2 (human)	Applied Biosystems	Hs00975961_g1
18S (Human)	Applied Biosystems	Hs99999901_s1
Il1b (mouse) FW (5′-3′) TGGCAACTGTTCCTG RV (5′-3′) GGAAGCAGCCCTTCATCTTT	This paper	N/A
Rps18 (mouse) FW (5′-3′) TGGGAACTTCTCATCCCTTTGRV (5′-3′) GGATGTGAAGGATGGGAAGT	This paper	N/A
Ifnb (mouse) FW (5′-3′) CCCTATGGAGATGACGGAGA RV (5′-3′) CCCAGTGCTGGAGAAATTGT	This paper	N/A
Ifit2 (mouse) FW (5′-3′) CCTGGATCAAGAATGGGCTA RV (5′-3′) CATCCCACGATCCAGAAACT	This paper	N/A