Figure 2.

Glucose sensing and transport genes are deregulated in the Cgyps1-11Δ mutant.A, MitoTracker Green–based mitochondrial morphology analysis. Representative maximum-intensity projection of Z-stack fluorescence confocal images showing mitochondrial network in YNB medium–grown, MitoTracker Green (100 nM)–stained log-phase cells of wt and Cgyps1-11Δ strains. The scale bar represents 2 μm. B, qRT-PCR-based expression analysis of indicated genes (two downregulated and four upregulated genes in the RNA-Seq experiment) in wt and Cgyps1-11Δ strains. Strains were grown to log-phase in YPD medium for 4 h, followed by RNA extraction using acid phenol. The cDNA synthesis reaction was set up with 500 ng total RNA, followed by qRT-PCR using appropriate primer sets, and gene transcript levels were measured using the 2^−ΔΔCt method. Please note that CgHXT2/10 (D) and CgHXT2/10 (I) refer to CAGL0D02640g/CAGL0D02662g and CAGL0I00286g genes, respectively. Data (mean ± SEM, n = 3–4) were normalized against CgACT1 mRNA control and represent fold change in expression in Cgyps1-11Δ cells, compared with wt cultures (considered as 1.0). ∗p ≤ 0.05; ∗∗p ≤ 0.01, paired two-tailed Student's t test. C, qRT-PCR-based expression analysis of indicated genes in YPD medium–grown log-phase Cgyps1Δ and Cgyps7Δ cells. Data (mean ± SD, n = 2–3) were normalized against CgACT1 mRNA control and represent fold change in expression in Cgyps1Δ and Cgyps7Δ cells, compared with wt cultures (considered as 1.0). D, qRT-PCR-based expression analysis of CgYPS1 and CgYPS7 genes in log-phase wt cells that were grown in YNB medium containing low (0.03%), regular (2%), and high (5%) glucose for 2 h. Data (mean ± SEM, n = 3–4) were normalized against CgACT1 mRNA control and represent fold change in expression in low-glucose and high-glucose medium–grown wt cells, compared with regular-glucose medium–grown wt cells (considered as 1.0). ∗p ≤ 0.05, paired two-tailed Student's t test. DIC, differential interference contrast.