Figure 4.

CgSNF3 gene loss confers growth advantage to Cgyps1-11Δ cells in medium containing 2% glucose. Time course analysis of wt, Cgyps1-11Δ, Cgsnf3Δ, and Cgsnf3Δyps1-11Δ strains. C. glabrata strains were grown overnight in YPD medium and inoculated at an initial A₆₀₀ of 0.1 in YNB medium containing 2% (A), 0.03% (B), 0.3% (C), and 5% (D) glucose. Cultures were incubated at 30 °C with continuous shaking (200 rpm), and absorbance was monitored at regular intervals till 48 h. The absorbance (A₆₀₀) values are plotted against time, and the growth period, corresponding to the log-phase (between 2 and 6 h), was used to determine the doubling time. Data represent mean ± SEM (n = 3–4). The one-way ANOVA with Tukey’s test was employed to determine the statistical significance of doubling time differences between strains. Red and black asterisks denote differences in doubling time between wt and mutants, and Cgyps1-11Δ and Cgsnf3Δyps1-11Δ mutants, respectively. ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001; ∗∗∗∗p ≤ 0.0001.