Skip to main content
. 2022 Feb 1;25(2):103847. doi: 10.1016/j.isci.2022.103847

Figure 3.

Figure 3

ex vivo analysis of OVA257-264 tetramer-positive CD8+ T cells in normal or NAFLD livers

(A–D) Frequency of OVA257-264 tetramer-positive CD8+ T cells from liver lymphocytes of mice given normal diet (ND) or methionine-choline-deficient (MCD) diet with or without doxycycline (dox). Low means low antigen expression due to no dox water while high means high antigen expression due to dox water. Data pooled from multiple experiments (B) Scatterplot with correlation of tumor weight from ND or MCD diet mice and percentage of tetramer (+) CD8+ T cells. Each diet group contains dox on and off. (C) Granzyme B (GRZb) staining of unstimulated OVA tetramer-positive CD8+ T cells from liver lymphocytes isolated from ND or MCD mice with or without Dox. (D) IFNg staining in OVA tetramer-positive CD8+ T cells after 4 h of stimulation with the OVA257-264 peptide.

(E) Degranulation assay of liver lymphocytes stimulated with Hep55.1c-Tet3G-LucOS tumor cells incubated with 1ug/mL of Dox.

(F) PD-1 staining of unstimulated OVA tetramer-positive CD8+ T cells from liver lymphocytes isolated from ND or MCD mice with or without Dox.

(G) CD44 staining of OVA tetramer-positive CD8+ T cells from liver lymphocytes isolated from ND or MCD mice with or without Dox.

(H) CD62L staining of OVA tetramer-positive CD8+ T cells from liver lymphocytes isolated from ND or MCD mice with or without Dox. All pooled data presented as mean with SD. One-way ANOVA with Tukey correction for (A) and (C)–(H). ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.