Macrophage suppression of OVA257-264-specific CD8+ T cells in normal or NAFLD livers. Immune cell monitoring showing
(A and B) cells per gram of liver tissue and (B) percentage of live lymphocytes isolated from mice given normal diet (ND) or methionine-choline-deficient (MCD) diet (NKT: natural killer T cell, Treg: T regulatory cell, MDSC: monocytic-myeloid-derived suppressor cell, DC: dendritic cell).
(C) Diagram of the experimental design for clodronate depletion.
(D and E) Excised tumors and (E) tumor to liver ratio of mice fed ND or an MCD diet with or without dox and clodronate 10 days after an intrahepatic injection of Hep55.1c-Tet3G-LucOS cells. Data pooled from multiple experiments. Low means low antigen expression due to no dox water while high means high antigen expression due to dox water. All pooled data presented as mean with SD. One-way ANOVA with Tukey correction for A and (F) ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.