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. 2021 Dec 16;2(2):100084. doi: 10.1016/j.xjidi.2021.100084

Figure 7.

Figure 7

In vitro T cell cytokine studies using patient-isolated cutaneous T cells. (a) Proliferative responses of human malignant T cells isolated from skin biopsies (MF06) to bacteria isolated from lesional compared with that from nonlesional (control) regions (2 inches next to each matched lesion). Y-axis indicates proliferation as RLUs using a nonradioactive ATP release assay. (b) Individual cytokine graphs with cytokine concentrations (in pg/ml) from the supernatant of the cutaneous T cells stimulated for 72 hours with heat-killed bacteria as indicated. Functional phenotypes were characterized by cytokine secretion of IL-21, GM-CSF, IFN-γ, TNF-α, IL-17A, IL-4, IL-5, IL-13, and IL-10, respectively. Data points represent duplicates. P-values were calculated using the unpaired two-tailed Student’s t-test. Significance levels are indicated by asterisks: ∗P < 0.05; ∗∗P < 0.01. ATP, adenosine triphosphate; MF, mycosis fungoides; RLU, reactive light unit.