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. 2021 Dec 16;2(2):100084. doi: 10.1016/j.xjidi.2021.100084

Figure 8.

Figure 8

In vitro T cell proliferation and cytokine studies using patient-isolated peripheral blood T cells. (a) Human CD4+ T cells isolated from patient peripheral blood (MF06) selected for skin-homing markers CCR4 and CLA show proliferation to Bacillus safensis compared with unstimulated T cells. Y-axis indicates proliferation as RLUs using a nonradioactive ATP release assay. (b, c) Cytokine concentrations (in pg/ml) from the supernatant of the cutaneous T cells stimulated for 72 hours with bacteria as indicated, represented as (b) Z-score and (c) individual graphs. Functional phenotypes were characterized by cytokine secretion of IL-21, GM-CSF, IFN-γ, TNF-α, IL-17A, IL-4, IL-5, IL-13, and IL-10, respectively. Data points represent duplicates. P-values were calculated using the unpaired two-tailed Student’s t-test. Significance levels are indicated by asterisks: ∗P < 0.05; ∗∗P < 0.01. ATP, adenosine triphosphate; CLA, cutaneous lymphocyte‒associated antigen; MF, mycosis fungoides; RLU, reactive light unit.