Figure 1.

Dissection of mouse oviducts

(A) Mouse oviducts are located by making a sagittal and transverse incision across the mouse’s abdomen to expose the peritoneal cavity.

(B) The gastrointestinal (GI) tract is then moved cranially and the fat pads caudally to reveal the uteri.

(C) The oviducts can be found adjacent to the ovaries by tracing the uteri laterally.

(D) An isolated oviduct attached to a portion of the uterus and ovary.

(E) A dissected and elongated oviduct after removal of ovary and connective tissue.

(F) After removal of the uterus.

(G) An example of segregation into distal and proximal regions with removal of the ampulla-isthmus junction (AIJ).

(H) Oviducts after being pulled apart into 1 mm fragments for enzymatic digestion. Scale bars = 1 mm. FRT = female reproductive tract, INF = infundibulum, AMP = ampulla, AIJ = ampulla-isthmus junction, IS = isthmus, UTJ = uterotubal junction.