Figure 2.

2D seeding and isolation densities

(A) Brightfield images showing 2D oviduct epithelial cultures after 24 h of culture. To ensure successful expansion of epithelial cells, cultures should be seeded at a density of 20,000 cells per 96-well plate.

(B) Brightfield images showing 2D oviduct epithelial cultures after 3 days of culture. 2D cultures should be transferred to 3D once at 75% confluency, as indicated in the central panel. Scale bars = 100 μm.