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. 2022 Feb 9;3(1):101164. doi: 10.1016/j.xpro.2022.101164

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Characterization of mouse oviduct organoids

(A) A brightfield image of mouse oviduct epithelial organoids after 7 days in culture.

(B) A whole mount confocal image of a mouse oviduct epithelial organoid after differentiation has been induced by notch inhibition (see step 6a). Multiciliated cells are labeled with FOXJ1 and cilia with Actub (acetylated tubulin). Nuclei are labeled with DAPI.

(C and D) Whole mount imaging of differentiated mouse oviduct epithelial organoids showing retention of oviduct epithelial markers PAX8, SOX17 and cilia labeled with Actub. Nuclei are labeled with DAPI. Scale bars = 100 μm.