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. 2022 Feb 9;3(1):101164. doi: 10.1016/j.xpro.2022.101164

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Viral transduction of mouse oviduct epithelial organoids

(A and B) Live images of mouse oviduct epithelial organoids generated from Tdtomato^Flox/Flox mice after transduction during 2D culture with a cre-expressing Adenovirus. Tdtomato expression was identified in some organoids indicating successful viral transduction.

(C and D) Live images of mouse oviduct epithelial organoids generated from Tdtomato^Flox/Flox mice after in situ transduction on day 7 of culture with a cre-expressing Adenovirus. Tdtomato expression was identified in a subpopulation of epithelial cells within some organoids, indicating successful viral transduction. Scale bars = 100 μm.