Growth factor and inhibitor stocks

Reagent	Final stock concentration	Amount	Volume
TGFBR1 kinase inhibitor IV (SB431542)	10 mM (20,000×)	5 mg	1.189 mL DMSO
Mouse fibroblast growth factor 10 (mFGF10)	100 μg/mL (1,000×)	25 μg	250 μL sterile PBS + 0.1% BSA
Human epidermal Growth Factor (hEGF)	500 μg/mL (5,000×)	500 μg	1 mL sterile PBS + 0.1% BSA
Human Noggin	200 μg/mL (2,000×)	1000 μg	5 mL sterile PBS + 0.1% BSA
Human R-spondin1	600 μg/mL (1,000×)	500 μg	833 μL sterile PBS + 0.1% BSA
Mouse Wnt family member 3A (mWnt3a)	100 μg/mL (1,000×)	10 μg	100 μL sterile PBS + 0.1% BSA
ROCK inhibitor (Y-27632)	10 mM (1,000×)	1 mg	312 μL sterile PBS + 0.1% BSA