Collagenase solution

Reagent	Final concentration	Amount
Dissection solution	1×	940 mL
Collagenase B (100 mg/mL)	5 mg/mL	50 μL
DNase I (1 U/μL)	0.01 U/μL	10 μL
Total	n/a	1 mL

Collagenase solution should be used on the same day and kept on ice.