Organoid mounting media

Reagent	Final concentration	Amount
10× PBS	1×	10 mL
Glycerol	90 %	89 mL
20 % n-propyl gallate (DMSO)	0.2 %	1 mL
Total	n/a	100 mL

Prepare a stock of 20 % n-propyl gallate by dissolving 1 g in 5 mL DMSO prior to making the mounting media. 1 mL of this solution should be slowly added to the thoroughly mixed PBS:Glycerol (1:9) solution dropwise, while simultaneously stirring rapidly to obtain a final concentration of 0.2%. Mounting media can be stored at 4°C in the dark for several months.