Figure 3.

Specific recognition of tumor cells and NK cells by CP-bi-apt

(A–C) Confocal fluorescence images of (A) SMMC-7721, (B) HeLa, and (C) NK cells incubated with Evagreen-labeled aptamers as indicated (200 nM). In block group, the cells were pre-treated with anti-PD-L1 antibody or anti-CD16 antibody and then incubated with CP-bi-apt. Data are presented as mean ± SEM (error bar); ∗∗∗∗p<0.0001. (D and E) Flow cytometric analysis of CFSE-labeled (D) SMMC-7721 cells or (E) HeLa cells after co-incubating with Cell-Tracker-Deep-Red-labeled NK cells (E:T = 1:1) and different aptamers (PD-L1-apt, CD16-apt, C_mutP-bi-apt, CP_mut-bi-apt, or CP-bi-apt) is shown.