Figure 6.

In vivo antitumor effect of different aptamers and adoptive NK cells

(A) The average growth curves of the tumors after various treatments (PBS, free NK cells, CD16-apt + NK cells, PD-L1-apt + NK cells, CD16-apt + PD-L1-apt + NK cells, and CP-bi-apt + NK cells; n = 5). (B–E) The ELISA analysis of (B) IFN-γ, (C) TNF-α, (D) granzyme B, and (E) perforin in tumors isolated from mice at day 3 after the last injection (n = 3). Data are presented as mean ± SEM; ∗∗p<0.01, ∗∗∗p<0.001, ∗∗∗∗p<0.0001. (F) The representative CD16 immunofluorescence staining of the infiltrated NK cells (red) in tumor . (G and H) Perforin (G) and granzyme B (H) immunofluorescence staining of the NK cells in tumors, and CD56 was used as the marker. Scale bars: 100 μm. The insets are the corresponding enlarged image in each condition.