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. 2022 Jan 25;25(2):103806. doi: 10.1016/j.isci.2022.103806

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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H₂O₂ stress pathway qPCR validation test

Correlation plot obtained from RT-qPCR results generated from L-EVs and Cell lysate mRNA isolated from control cells (None), TRACE construct eGFP-EIFE4-C-YTHDF1/GPB1-CD9 (4EC1) stable cell line on eight genes corresponding to the oxidative stress pathway.

(A) Batch of H₂O₂ stressed cells and Batch of unstressed cells correlation cell lysates vs L-EVs.

(B) Correlation plots for differentially expressed candidate genes induced by oxidative stress (compared to baseline) in 4EC1 transfected or control cells. Results obtained from triplicate cell populations, 15M cells per condition (See also Figure S14)