| Component | Volume (μL) | Final concentration |
|---|---|---|
| RNA reaction mix SuperScript II (200 U μL−1) |
11 1 |
– 100 U |
| RNAse Inhibitor (40 U μL−1) | 0.5 | 10 U |
| SuperSript II Buffer (5X) | 4 | 1X |
| DTT (100 mM) | 1 | 5 mM |
| Betaine (5 M) | 2 | 0.5 M |
| MgCl2 (1 M) | 0.1 | 5 mM |
| TSO (100 μM) | 0.2 | 1 μM |
| NRAse free water | 0.2 | – |
| Total volume | 20 | – |
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