Fig. 1.

SelK is upregulated in during SCs differentiation and C2C12 myoblast differentiation. (A) Immunofluorescence staining of Pax7 (red) and SelK (green) in TA muscle of wild-type mice without 1.2% BaCl₂ injection. Scale bar = 50 μm. (B) Immunofluorescence staining of Pax7 (red) and SelK (green) in TA muscle of wild-type mice at 5 d postinjury. Scale bar = 50 μm. (C) Immunofluorescence staining of MyoG (red) and SelK (green) in TA muscles of wild-type mice at 5 d postinjury. Scale bar = 50 μm. (D) Western blot analysis of SelK protein levels in uninjured and 5 d-injured TA muscle. N = 3 mice in each group for A-D. (E) Immunofluorescence staining of SelK in C2C12 myoblasts cultured in growth medium for 1 d or in differentiation medium for 2 d. Scale bar = 50 μm. (F) qRT-PCR analysis of SelK mRNA levels in C2C12 myoblasts cultured in growth medium for 1 or 2 d (G1 or G2) or in differentiation medium for 1, 3, 5 or 7 d (D1, D3, D5 or D7). (G) and (H) Western blotting analysis of SelK protein levels in C2C12 myoblasts cultured in growth or differentiation medium for 1 or 2 d (G1 or G2) or in differentiation medium for 1, 3, 5 or 7 d (D1, D3, D5 or D7). N = 3 in each group for E-H. The results are presented as means ± S.D. ***p < 0.001, values significantly different from the corresponding control by unpaired t-test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)