Fig. 3.

Inactivation of SelK inhibits the myogenic differentiation progression of SCs. (A) Immunofluorescence staining of MyoD (green) and Pax7 (red) in TA muscle of Ctrl and SelK KO mice at 3 d postinjury (N = 3). Nuclei were labelled using DAPI staining. Scale bar = 20 μm. (B) Quantification of the percentages of Pax7⁺MyoD⁻, Pax7⁺MyoD⁺ and Pax7⁻MyoD⁺ cell populations in Ctrl and SelK KO TA muscles at 3 d postinjury. (C) Immunofluorescence staining of MyoD (green) and MyoG (red) in TA muscle of Ctrl and SelK KO mice at 5 d postinjury (N = 3). Nuclei were labelled using DAPI staining. Scale bar = 20 μm. (D) Quantification of the percentages of MyoD⁺MyoG⁻, MyoD⁺MyoG⁺ and MyoD⁻MyoG⁺ cell populations in Ctrl and SelK KO TA muscles at 5 d postinjury. (E) qRT-PCR analysis of myogenic transcription factors (MyoD1, MyoG, MyH1, MyH2 and MyH3) mRNA levels in TA muscle of Ctrl and SelK KO mice at 5 d postinjury (N = 3). (F) and (G) Western blotting analysis of MyoD, MyoG and MyHC protein levels in uninjured and 5 d-postinjured TA muscle of Ctrl and SelK KO mice (N = 3). The results are presented as means ± S.D. *p < 0.05, **p < 0.005, ***p < 0.001, values significantly different from the corresponding control by unpaired t-test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)