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. 2022 Feb 4;50:102255. doi: 10.1016/j.redox.2022.102255

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — The differentiation ability of C2C12 myoblast cells is reduced by SelK silencing. (A) and (B) Western blotting analysis of SelK protein levels in siSelK-transfected C2C12 myoblast cells cultured in differentiation medium for 1, 3 and 5 d (N = 3). (C) qRT-PCR analysis of SelK mRNA levels in siSelK-transfected C2C12 myoblast cells cultured in differentiation medium for 0, 1, 3 and 5 d (N = 3). (D) Immunofluorescence staining of MyHC in control siRNA (siNC) and SelK siRNA (siSelK) C2C12 myoblast cells cultured in differentiation medium for 5 d (N = 3). Scale bar = 100 μm. (E) Quantitative analysis of the percentage of MyHC⁺ C2C12 myoblast cells. (F) Immunofluorescence staining of MyoG in siNC and siSelK C2C12 myoblast cells cultured in differentiation medium for 5 d (N = 3). Scale bar = 50 μm. (G) Quantitative analysis of the percentage of MyoG⁺ C2C12 myoblast cells. (H) and (I) Western blotting analysis of MyoD, MyoG and MyHC protein levels in siNC and siSelK C2C12 myoblast cells cultured in differentiation medium for 0, 1, 3 and 5 d (N = 3). The results are presented as means ± S.D. **p < 0.005, ***p < 0.001, values significantly different from the corresponding control by unpaired t-test.