Fig. 7.

Silencing of SelK enhances oxidative stress in SCs and C2C12 myoblast cells during myogenic differentiation. (A) Activities of antioxidant enzymes (SOD and CAT) and the contents of oxidative stress markers (H₂O₂, MDA and T-AOC) in TA muscles of Ctrl and SelK KO mice at 5 d postinjury (N = 3). (B) and (C) DCFH-DA staining and quantification of ROS generation in C2C12 myoblast cells cultured in differentiation medium for 0, 1, 3 and 5 d (N = 3). (D) Immunofluorescence staining of MyHC and MyoG and bright field microscopy in siNC and siSelK C2C12 myoblast cells treated with NAC or PBS after 5 d in differentiation medium (N = 3). (E) Quantitative analysis of the percentage of MyHC⁺ cells and (F) the percentage of MyoG⁺ cells treated with NAC or PBS after 5 d in differentiation medium. (G) Immunofluorescence staining of MyHC and MyoG and bright field microscopy in siNC and siSelK C2C12 myoblast cells treated with SF or DMSO after 5 d in differentiation medium (N = 3). (H) Quantitative analysis of the percentage of MyHC⁺ cells and (I) the percentage of MyoG⁺ cells treated with SF or DMSO after 5 d in differentiation medium. (J) and (K) Western blotting analysis of MyoD, MyoG and MyHC protein levels in siNC and siSelK C2C12 myoblast cells treated with NAC or PBS after 5 d in differentiation medium (N = 3). (L) and (M) Western blotting analysis of MyoD, MyoG and MyHC protein levels in siNC and siSelK C2C12 myoblast cells treated with SF or DMSO after 5 d in differentiation medium (N = 3). The results are presented as means ± S.D. *p < 0.05, **p < 0.005, ***p < 0.001, values significantly different from the corresponding control by unpaired t-test. Bars that do not share the same letters are significantly different (p < 0.05) from each other by one-way ANOVA.