Isolation and characterization of Exos from culture supernatant of HUVECs, PD-L1 overexpressed HUVECs, and PD-L1 knockout HUVECs. (A) The size distribution of HUVEC@Exos, HUVECPD−L1@Exos and HUVECPD−L1-/-@Exos was measured DLS. (B) Represent image of the three kinds of Exos were achieved by TEM. Scale Bar: 100 nm. (C) The protein levels of PD-L1, CD81 and TSG101 in these kinds of exosomes. (D) The PD-L1 expression in each group was measured by qRT-PCR analysis. *P < 0.05; **P < 0.01, ***P < 0.001.