Table 2.

Parameters derived from SAXS analysis of Csa3_Sso

Sample	Concentration (mg/ml)	Guinier		GNOM		Pxa	MM (kDa)b
		qRg	Rg (Å)c	Rg (Å)	Dmax (Å)
Csa3Sso	8.3	0.38–1.23	28.7 ± 0.1	28.2 ± 0.1	100	3.9	58.9 (56)
	6.7	0.23–1.28	28.4 ± 0.1	28.1 ± 0.2	101	3.9	58.9 (56)
Csa3Sso + 180 μM cA4	5.5	0.40–1.26	30.8 ± 0.2	30.9 ± 0.2	110	4.0	66.1 (56)
	4.4	0.44–1.34	31.3 ± 0.2	31.2 ± 0.3	110	4.0	66.4 (56)

^aPorod exponent (P_x). Values near ∼4 indicate compactness, whereas lower values between 2 and 3 indicate significant lack of compactness and increased volumes (105). These values were determined using the program ScÅtter (https://bl1231.als.lbl.gov/scatter/).

^bMass determinations using the Q_r invariant (106) were determined using the program RAW. Expected dimeric mass is shown in parentheses.

^cErrors reported reflect the uncertainty in the value for R_g determined using classical Guinier fitting.