Table 2.

Results of single concentration developmental toxicity testing.

Structural Class	PFAS	CFC	Conc (μM)	Mort 24	Mort 120	EPR			LPR			LSR		n (LPR/LSR)
						B	E	R	Light	Dark	AUC	Peak	Controls	Treated
Carboxylic acids	PFBA	3	1.17						HYPER				31	32
	PFPeA	4	0.95						HYPO				31	29
	PFHxA	5	0.80								HYPER		30	32
	PFHpA	6	0.69										30	32
	PFOA	7	0.60										31/32	32
	PFNA	8	0.54									HYPO	31/32	32
	PFDA	19	0.49										31	30/32
	PFUdA	10	0.44										31	30
	PFDoA	11	0.41						HYPER				32	31
	PFTrDA	12	0.38										32	31
	PFTeDA	13	0.35									HYPO	32	31
	PFHxDA	15	0.31										32	29
	PFODA	17	0.27						HYPER				32	32
Fluorotelomer carboxylic acids	FPrPA	3	1.03										28	30
	FPePA	5	0.73										31	29
	FHpPA	7	0.57						HYPO				31	30
Unsaturated fluorotelomer carboxylic acids	FHUEA	6	0.70										30	29
	FOUEA	8	0.55										30	29
Ether/polyether carboxylic acids	HFPO-DA	3,2	0.76						HYPO				32	31
	NaDONA	1,3,2	0.62										32	30
Sulfonic acids	PFPrS	3	0.92						HYPO				28	29
	PFBS	4	0.74						HYPER				27	28
	PFPeS	5	0.67						HYPER				27	27
	PFHxS	6	0.57										28	27
	PFHpS	7	0.53						HYPO				28	26
	PFOS	8	0.46						HYPO				29	29
	8-Cl PFOS	8	0.46						HYPO				30	29
	PFNS	9	0.44										24	26
	PFDS	10	0.40										30	31
	PFDoS	11	0.35										31	30
Sulfonic acids (cyclic)	PFECHS	8	0.50									HYPO	29	31
Fluorotelomer sulfonic acids	4:2 FTS	4	0.71										31	30
	6:2 FTS	6	0.56										31	32
	8:2 FTS	8	0.45										28	29
	10:2 FTS	10	0.38										31	30
Sulfonamides	N-Methyl FOSA	8	0.49										30	31
	N-Ethyl FOSA	8	0.47										30	30
Sulfonamidoacetic acids	FOSAA	8	0.45										27	28
	N-Methyl FOSAA	8	0.44										27	27
	N-Ethyl FOSAA	8	0.43										27	25
Chlorinated ether sulfonic acids	9-Cl PF3ONS	6,2	0.44										27	27
	11-Cl PF3OUdS	8,2	0.37										29	32
Phosphonic acids	PFHxPA	6	0.62						HYPO				32	30
	Cl PFHxPA *	6	0.40										28	28
	PFOPA	8	0.50										30	31
	Cl PFOPA	8	0.48						HYPER				25	25
	PFDPA	10	0.42										32	31
Phosphate ethers (mono-/di-substituted)	6:2 PAP	6	0.51										29	28
	8:2 PAP	8	0.43										29	23
	SAMPAP	8	0.36										30	30
	6:2 DiPAP	6,6	0.31										28	29
	8:2 DiPAP	8,8	0.25						HYPER				28	31
	DiSAMPAP	8,8	0.20							HYPER			28	26
Phosphinic acids	6:6 PFPiA	6,6	0.35						HYPO				27	25
	8:8 PFPiA	8,8	0.27										27	26
Zwitterions	N-CMAMP	6	0.44										24	24
	N-AP-FHxSA	6	0.52										26	28
	N-TAMP-FHxSA	6	0.50										26	31
Mixtures	PFC-MXA	-	0.27**										28	27
	Mixture B	-	0.023**						HYPER				28	25

CFC = Continuously fluorinated carbons; Conc = Concentration; Mort = Mortality (at 24 and 120 hpf, respectively); EPR = Embryonic Photomotor Response: B = Background, E = Excitatory, R = Refractory period; LPR = Larval Photomotor Response (light and dark periods, respectively); LSR = Larval Startle Response: AUC = Area Under the Curve analysis, Peak = peak analysis; n (LPR/LSR) = Sample sizes of the control and treated groups; Results: HYPER = hyperactive (yellow), HYPO = hypoactive (blue) compared to controls. Grey coloring indicates no significant effect.

^*Tested at half the target concentration due to dilution error (0.4 μM).

^**Concentration describes ∑PFAS in mixture.