Table 1.
Summary of RiboTag IP capture for eL22-HA, Th mRNA, and Slc6a3/DAT mRNA
| Percentage of total yield | ||||
|---|---|---|---|---|
|
| ||||
| Region | eL22-HA protein | Th mRNA |
Slc6a3/DAT mRNA |
|
| SNc | 37.4 ± 8.2% | 25.5 ± 6.4% | 42.0 ± 5.2% | |
| VTA | 54.5 ± 8.8% | 69.6 ± 5.4% | 52.9 ± 4.1% | |
| SNr | 4.13 ± 0.77% | 4.0 ± 1.2% | 4.62 ± 1.02% | |
| Striatum | 2.23 ± 0.33% | 0.14 ± 0.07% | Undetectable | |
| Striatum +1% VM spike-in | 1.75 ± 1.24% | 0.78 ± 0.52% | 0.51 ± 0.29% | |
| TH (Th) mRNA | ||||
|
| ||||
| Region | Log2 (ΔYield) Cre+ - Cre− | Tukey HSD (p-adj) | Log2 FC (IP/Input) Cre+ - Cre− | Tukey HSD (p-adj) |
|
| ||||
| SNc | 7.88 ± 1.16 | <0.001 | 2.88 ± 0.44 | 0.0013 |
| VTA | 10.49 ± 0.73 | <0.001 | 4.28 ± 0.52 | <0.001 |
| SNr | 6.93 ± 1.03 | <0.001 | 6.82 ± 0.73 | <0.001 |
| Striatum | −1.16 ± 1.25 | >0.9 | 0.20 ± 0.50 | >0.9 |
| Striatum +1% VM spike-in | 1.94 ± 0.94 | NA | 5.89 ± 0.40 | NA |
| Dopamine transporter (Slc6a3/DAT) mRNA | ||||
|
| ||||
| Region | Log2 (ΔYield) Cre+/Cre− | Tukey HSD (p-adj) | Log2 FC (IP/Input) Cre+ - Cre− | Tukey HSD (p-adj) |
|
| ||||
| SNc | 8.41 ± 1.30 | <0.001 | 3.62 ± 0.51 | <0.001 |
| VTA | 9.65 ± 0.78 | <0.001 | 3.54 ± 0.22 | <0.001 |
| SNr | 7.45 ± 0.48 | <0.001 | 7.48 ± 0.78 | <0.001 |
| Striatum | NA | NA | NA | NA |
| Striatum +1% VM spike-in | Cre− undetectable | NA | 7.21 ± 0.44 (Cre+) Cre− undetectable | NA |
Upper: Mean percentage of total yield ±SEM for each region across all Cre+ RiboTag IPs. Data from Figures 2B–2D (eL22-HA protein, n = 3 each region) and Figures 2E and 2F (Th and Slc6a3 mRNA, n = 3–4 each genotype/region).
Lower: Mean log2 differences in yield (left) or enrichment (right) ± SEM between Cre+ and Cre− RiboTag IPs for each region/mRNA. Data and p-adj from Tukey’s HSD post hoc comparisons are from Figures 2E and 2F (Th and Slc6a3 mRNA, n = 3–4 each genotype/region).