Figure 2.

Illustration of steps involved in establishing primary cell cultures from human skin

(A) Dishes arranged in tissue culture hood for decontamination and rinsing of skin tissue, showing dishes containing 5% Dettol (whitish solution) and HBS rinses (pinkish solution).

(B) Cutting skin into strips measuring ∼2 cm × 0.25 cm after trimming and decontaminating, and prior to Dispase digestion.

(C) Separation of epidermis from dermis following overnight (∼16 h) Dispase digestion.

(D) Strips of dermis (left) and epidermis (right) after Dispase digestion and separation.

(E) Extrusion of HDMEC from dermal strips.

(F) Mincing of dermal strips prior to collagenase digestion.

(G) Suspension of minced dermis in collagenase.

(H) T150 flask containing dermal fibroblasts and dermal “pieces” following collagenase digestion, rinsing, and inoculation.

(I) Filtration of digested epidermal tissue through cell strainer following trypsin treatment.