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. 2022 Feb 10;3(1):101172. doi: 10.1016/j.xpro.2022.101172

Figure 4.

Figure 4

Images of P0-P1 primary human dermal microvascular endothelial cells (HDMECs) in culture

(A) Image of HDMEC P0 culture on day 1 after initiation. Note the appearance of cells associated with residual pieces of tissue. HDMECs are relatively compact at this stage and tightly associated with each other.

(B) Image of the same HDMEC P0 culture on day 4 after initiation. Note that the HDMECs now appear elongated but remain tightly associated in colonies. A contaminating fibroblast is observed (arrow).

(C) HDMEC at P0 culture day 6.

(D) HDMEC at P0 culture day 8, at time of harvest for passaging. White arrows indicate contaminating fibroblasts.

(E) P1 HDMEC, one day after passaging.

(F) P1 HDMEC at day 5. Contaminating fibroblasts are occasionally observed (white arrows in B, D, E, F). These tend to have more irregular shapes than HDMEC and are usually present as individual cells rather than colonies. Original image magnifications: 10× (A, B); 4× (C-F).