Figure 5.

Images of P0-P1 primary human fibroblasts in culture

(A) P0 fibroblasts in #1 flask at culture day 2, after removal of tissue “pieces.”

(B) P0 fibroblasts in #1 flasks at culture day 4.

(C) P0 fibroblasts in #2 flask on culture day 4, 3 days after removal from #1 flasks.

(D) P0 fibroblasts in #2 flasks on day 5, 4 days after removal from #1 flasks, at time of harvest for cryopreservation and/or passaging.

(E) P1 fibroblasts, 1 day after passage and inoculation.

(F) P1 fibroblasts, 5 days after inoculation, one day prior to planned harvest. Original image magnifications: 10× (A, B); 4× (C–F).