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Recommended inoculation densities for different primary cell types
| Cell type | Density (cells/cm2) | Approximate time to reach ∼90% confluence |
|---|---|---|
| Keratinocytes | 2,000 | 6–7 days |
| 5,000 | 5–6 days | |
| 10,000 | 4–5 days | |
| Fibroblasts | 2,000 | 5–6 days |
| 5,000 | 4–5 days | |
| 10,000 | 3–4 days | |
| Melanocytesa | 5,000 | 6–7 days |
| 10,000 | 5–6 days | |
| Human Dermal Microvascular Endothelial Cells (HDMEC)a | 5,000 | 6–7 days |
| 10,000 | 5–6 days |
For melanocytes and HDMEC, we do not recommend inoculating into flasks at <5,000/cm2.
