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| Component | Final concentration, mM | Amount to add for 10 L |
|---|---|---|
| HEPES | 30.00 | 71.49 g |
| Phenol red (3.3 mM stock solution)∗ | 3.30E-3 | 10 mL |
| Dextrose | 10.00 | 18.02 g |
| Potassium chloride | 3.00 | 2.24 g |
| Sodium chloride | 131.71 | 76.97 g |
| Sodium phosphate heptahydrate | 1.00 | 2.68 g |
∗To prepare 3.3 mM phenol red stock solution, dissolve 0.62 g phenol red in Milli-Q water, bring to 500 mL final volume, filter sterilize and store at room temperature (20°C–22°C) for up to one year.
