REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CD31 antibody (mouse monoclonal)	BD Pharmingen	Cat#555444; RRID: AB_395837
Anti-Cytokeratin 14 antibody (rabbit monoclonal)	Novus Biologicals	Cat#NBP2-67585; RRID: AB_2809501
Anti-TYRP1 antibody (mouse monoclonal)	BioLegend	Cat#SIG-38150; RRID: AB_10175227
Anti-Vimentin antibody (rabbit polyclonal)	Abcepta	Cat#AP2739a; RRID: AB_2216120
Donkey-anti-Mouse IgG (H+L), Alexa Fluor 594	Fisher Scientific	Cat#A21203; RRID: AB_141633
Donkey-anti-Rabbit IgG (H+L), Alexa Fluor 594	Fisher Scientific	Cat#A21207; RRID: AB_141637
Chemicals, peptides, and recombinant proteins
HEPES	Sigma-Aldrich	Cat#H4034
Dextrose	Fisher Scientific	Cat#D16-500
Potassium chloride	Fisher Scientific	Cat#P217
Sodium chloride	Fisher Scientific	Cat#S271
Sodium phosphate dibasic anhydrous	Fisher Scientific	Cat#S375
Sodium phosphate monobasic monohydrate	Fisher Scientific	Cat#S468
Calcium chloride dihydrate	Fisher Scientific	Cat#C79
Sodium bicarbonate	Fisher Scientific	Cat#S233
Gibco™ Medium 154CF, Kit	Fisher Scientific	Cat#M154CF500
Gibco™ Human Keratinocyte Growth Supplement	Fisher Scientific	Cat#S0015
DMEM, powder, low glucose, pyruvate	Fisher Scientific	Cat#31-600-091
Gibco Fetal Bovine Serum, Certified	Fisher Scientific	Cat#16-000-044
Medium 131	Sigma-Aldrich	Cat#M8537-10L
Microvascular Growth Supplement (MVGS)	Fisher Scientific	Cat#S00525
Attachment Factor	Fisher Scientific	Cat#S006100
Epidermal growth factor (EGF), recombinant human	PeproTech	Cat#AF-100-15
Hydrocortisone	Sigma-Aldrich	Cat#H4001
Insulin, recombinant human	Sigma-Aldrich	Cat#I2643
Bovine pituitary extract	Hammond Cell Tech	Cat#1078-NZ
Penicillin/Streptomycin/Fungizone (PSF); Gibco Antibiotic-Antimycotic (100×)	Fisher Scientific	Cat#15-240-096
Triiodothyronine	Sigma-Aldrich	Cat#T5516
α-Melanocyte stimulating hormone (αMSH)	Sigma-Aldrich	Cat#M4135
Endothelin 1	Sigma-Aldrich	Cat#E7764
Basic fibroblast growth factor (bFGF)	PeproTech	Cat#100-18B
Ascorbic acid 2-phosphate (AA2P)	Sigma-Aldrich	Cat#A8960
Collagen I-Cell Culture Surface Coating Kit	ScienCell Research Laboratories	Cat#8188
Trypsin	Sigma-Aldrich	Cat#T8003
Ethylenediaminetetraacetic acid (EDTA), anhydrous	Sigma-Aldrich	Cat#EDS
0.5 M EDTA solution	Sigma-Aldrich	Cat#E7889
Dettol Liquid First Aid Antiseptic (4.8% Chloroxylenol)	Reckitt Benckiser	Cat#PL 44/5011
Dispase II	Fisher Scientific	Cat#04942078001
Collagenase, Type I	Worthington Biochemical Corp.	Cat#LS004196
Lysol Disinfectant Solution	Andwin Scientific	Cat#21899-598
Dimethyl sulfoxide (DMSO)	Sigma Aldrich	Cat#D2650
Clear Bath	Spectrum Chemical	Cat#105540
Other
Vectashield Plus Mounting Medium with DAPI	Vector Laboratories	Cat#H-2000
Weck-Prep Blades (carbon steel razor blades)	Fisher Scientific	Cat#50-949-469
Sterile 0.22 μm Millipak Disposable Filter Units	Fisher Scientific	Cat#MPGL06GH2
PYREX™ Reusable Media Storage Bottles	Fisher Scientific	Cat#06-414-1D
Corning Centrifuge Tube Top Filters, 0.22 μm	Fisher Scientific	Cat#09-761-34
Corning Centrifuge Tube Top Filters, 0.45 μm	Fisher Scientific	Cat#09-761-35
Falcon 70 μm Cell Strainer	Fisher Scientific	Cat#08-771-2
Cryovials, 2 mL	Fisher Scientific	Cat#03-374-059
50 mL Conical tubes (polypropylene)	Fisher Scientific	Cat#06-443-21
pH Meter
Magnetic stir plate(s)
Water purification system, Milli-Q, Synthesis A10 or similar
Autoclave
Nalgene Polypropylene media storage bottles (optional)	United States Plastic Corp.	Cat#73107
Analytical balance and weigh boats
Peristaltic pump for high volume liquid filtration
Biosafety cabinet (tissue culture hood; Class II type A2)
Phase-contrast microscope
Tissue-culture treated flasks (T75, T150, and T225 sizes)	Corning	Cat#431082 (T225 size)
Serological pipets: sterile, individually wrapped, sizes ranging from 2 mL–50 mL
Aspirating pipets: sterile, individually wrapped plastic or sterilized glass Pasteur pipets
Forceps: fine tip and blunt tip
Hemostat/clamp
Curved scissors
Sterile scalpel blades and handle
Glass bead sterilizer; e.g., Braintree Scientific Germinator 500	Fisher Scientific	Cat#NC9956482
Aspirating flask with long tubing, for liquid waste
Refrigerated centrifuge for spinning 50 mL conical tubes
Water-jacketed CO2 incubator with saturated humidity (water pan containing deionized water with 5% Lysol) set to 37°C, 5% CO2
Water bath at 37°C for thawing/warming media & reagents