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. 2022 Feb 10;3(1):101160. doi: 10.1016/j.xpro.2022.101160

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Perfusion and extraction of the rat brain

(A) Overview of tools needed for the perfusion and extraction of rat brains. The name and identity of all tools can be found in the key resource table.

(B) Schematic depiction of intraventricular perfusion of the rat heart. Perfusion needle must be inserted into the left ventricle, while the right atrium needs to be incised to allow the blood to flow out.

(C) Prior to cryosectioning, brains need to be cut with a razorblade to separate the forebrain from the hindbrain/brainstem. If available, use a coronal brain matrix to separate the brain. The bottom end of the brain will be glued onto a specimen disk using Tissue-Tek^TM O.C.T.^TM compound. This figure was created with BioRender.com.