Figure 6.

Separation of fused astrocytes by cutting through astrocyte processes

(A) Occasionally, Imaris accidentally recognizes two separate astrocytes as one entity (yellow astrocytes), when their filaments are very close together. If this happens, use the cut function (shift + left-click in the pencil mode) and a thin blue line (the line was artificially enhanced for better visibility in this image) will appear. This line indicates where the cut will be made (red arrows). The blue lines in the original image are shown in the enlarged inset on the right. (B) Once the cut has been made, astrocytes will be separated and clicking on them will only select one (see single astrocyte highlighted in yellow), but no longer both.