Required tools for Cryosectioning (Part 3):

Setup	Surgical instruments	Consumable
•Hybridization oven •Slide rack •Slide tray •PAP pen •Humidifying paper •−80°C freezer •Water bath •Netwells •Well plates	•Forceps	•Acdbio multiplex V2 in situ hybridization kit •Wash buffer (acdbio) •dH2O •EtOH •0.01M PBS •Mounting media •RNAse zap wipes •Kimwipes •SuperFrostTM glass slides

All images presented in Part 5 (Confocal microscopy) were obtained using the Zeiss LSM 780 (upright) confocal microscope (Zen Black software, 2.1) using the following settings: