REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
GFAP, goat polyclonal, 1:500	Abcam	ab53554
Glutamine synthetase, mouse monoclonal, 1:500	Merck Millipore	MAB 302, clone GS-6
Chemicals, peptides, and recombinant proteins
Formaldehyde 37%	Sigma-Aldrich	8187081000
InvitrogenTM Nuclease-free water	Fisher Scientific	AM9930
Veterinary Euthasol Solution 100mL Pentobarbital Sodium Vial	Henry Schein	Cat#1311760
Wash buffer	Acdbio	Comes with Multiplex V2 RNAScope kit
VECTASHIELD® Antifade Mounting Medium with DAPI	Vector Laboratories	H-1200-10
Critical commercial assays
RNAscope Multiplex Fluorescent Reagent Kit v2	Acdbio	Cat#323100
Oxytocin receptor (OTR) probe (rat)	Acdbio	Cat#483671
Opal dye 570 Reagent pack (Cy3) or TSA dye (Cy3/Cy5)	Akoya Biosciences	FP1488001KT Or NEL745001KT
Experimental models: Organisms/strains
Wistar rat, wildtype female, adult, 10–14 weeks	Envigo Harlan	RccHan®:WIST
C57BL/6 mice, wildtype female, adult, 10–14 weeks	Envigo Harlan	C57BL/6JOlaHsd
Software and algorithms
Imaris 9.51 (or higher) for Neuroscientists	Oxford Instruments, Bitplane	https://imaris.oxinst.com/microscopy-imaging-software-free-trial
Zen Black Software 2.1	Zeiss	https://www.zeiss.de/mikroskopie/produkte/mikroskopsoftware/zen-lite/zen-lite-download.html
Other
SuperfrostTM Plus slides	Fisher scientific	Cat#10149870
CorningTM netwellsTM inserts	Merck	CLS3477-48AE
Fine bone trimmer	Roboz	RS-8469
Surgical scissors	Fine Science Tools	Cat#14001-18
Bone scissor	Fine Science Tools.	Cat#16030-12
Fine scissors	Fine Science Tools.	Cat#14058-11
Specimen discs 30mm	Leica Biosystems	Cat#14037008587
Two-stop peristaltic perfusion pump	Cole-Parmer	GZ-07522-20
Short syringe needle, 16 or 18 G	BD	305197 or 305195
Scintillation vials	Merck – Sigma-Aldrich	V7005
RNAseZapTM wipes	Thermo Fisher Scientific	AM9786
500mL beaker with handle and spout	Corning	1015P-500
Hybridization oven	Acdbio	Comes with Multiplex V2 RNAScope kit
Slide rack	Acdbio	Comes with Multiplex V2 RNAScope kit
Slide tray	Acdbio	Comes with Multiplex V2 RNAScope kit
PAP pen	Acdbio	Comes with Multiplex V2 RNAScope kit
Humidifying paper	Acdbio	Comes with Multiplex V2 RNAScope kit
Corning®Netwells®	Merck – Sigma-Aldrich	CLS3477
6-Well plates	Merck – Sigma-Aldrich	CC302/CLS3335
Confocal Microscope	Zeiss	LSM780