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NAC, Mito-Tempo and 4-ABAH-inhibitable ROS are responsible for oxidation and proteolysis of fibrinogen in TNFα-activated leukocytes. TNFα-activated leukocytes from healthy donors (n = 6) were incubated with indicated various antioxidants as described in Fig. 4 for indicated time periods. (A-F) Conditioned media was analyzed by OxyBlot and immunoblotting using fibrinogen Aα, Bβ and γ – specific antibodies. Data images are representative of 3 independent experiments.
