FIG 1.

Rom susceptibility and growth of farE deletion mutants. (A) Overview of the strategy used to generate the ΔfarE deletion mutants in HG001 and RomR strains. The delta symbol in the sequence indicates a deleted region. (B) Rom’s antimicrobial activity can be abrogated by addition of culture supernatant of HG001 and the RomR mutant from the end of the exponential phase. Growth of S. aureus HG001 was inhibited by Rom (2 μg/mL) (compare tubes 1 and 2). The addition of 20-times-concentrated supernatant (1:20 [vol/vol]) of HG001 (tube 3) and 2-times-concentrated supernatant (1:2 [vol/vol]) of RomR (tube 4) rescued the growth, while supernatants alone (tubes 5 and 6) had no impact on growth. Cells were grown in BM for 12 h.