FIG 6.

MrpC, DmxA, PmxA-mVenus, c-di-GMP, and cGAMP accumulation in aggregated and nonaggregated cells. (A) MrpC, DmxB, and PmxA-mVenus accumulate at the same levels in aggregated (A) and nonaggregated (NA) cells. Cells were harvested at the indicated time points of development and separated into the two cell fractions. A total of 10 μg of protein was loaded per lane, and samples were separated by SDS-PAGE. Top blots were probed with α-MrpC, α-DmxB, or α-GFP; middle blots with α-PilC; and bottom blots with α-Protein C antibodies. The PilC blots served as loading controls and the Protein C blots as cell separation controls. (B and C) c-di-GMP (B) and cGAMP (C) accumulate at the same levels in aggregated and nonaggregated cells of WT. Samples were generated as in panel A. Levels are shown as mean ± SD calculated from three biological replicates. Individual data points are in light blue. At each specific time point, no significant differences were identified in pairwise comparisons in Student’s t test.