Figure EV2. SNF1 complex is required for RPA enrichment on early origins.

• A
  Snap shot of Rfa1 ChIP‐Seq at Chromosome III. Cells were synchronized in G1 and released into the fresh medium containing 200 mM HU for 30 min. The sequencing reads were mapped to the yeast reference genome. The red dashed box indicated the relative read intensity of ARS305.
• B, C
  SNF1 is required for efficient recruitment of Rfa1 to HU‐stalled replication forks, which is independent on cell cycle. Cells were grown and synchronized in G1 by α‐factor before release into the fresh medium containing 2% (B) or 5% (C) glucose supplemented with 200 mM HU for the indicated time. Cell extracts were prepared and subjected to MYC–ChIP of Rfa1–13MYC. The amounts of DNA in the precipitates were quantified by qPCR. Error bars represent SD from three biological repeats.