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. 2022 Jan 14;41(4):e108290. doi: 10.15252/embj.2021108290

Figure 6. Zwf1 is the principal target of Mig1 in replication stress response.

Figure 6

  1. ZWF1 is required for snf1Δmig1Δ growing in HU. Overnight cultures were spotted onto plates containing HU, and cultivated at 30°C for 48 h before photography. Yeast spot assays were performed as in Fig 2A.
  2. Rfa1–GFP foci were measured as in Fig 3. At least 200 cells were analyzed for each experiment of three independent experiments. Error bars represent SD from three biological repeats. Statistical significance was evaluated based on Student’s t‐test (*0.01 < P‐values < 0.05).
  3. Rfa1–13MYC ChIP assays after HU treatment. Strains were synchronized in G1 phase by α‐factor prior to release into fresh media containing 200 mM HU for the indicated time points. Cell extracts were prepared as in Fig 3. Error bars represent SD from three biological repeats.
  4. Phosphorylation of Mig1 T489 S495 completely restores the ZWF1 mRNA levels in snf1Δ. The experiments were done as in Fig 1. Error bars represent SD from three biological repeats. Statistical significance was evaluated based on Student’s t‐test (*0.01 < P‐values < 0.05; **0.001 < P‐values < 0.01).