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A
Reduced weight (in grams) of adult Rb∆K7 (n = 11) vs. control littermate (n = 17) males. Results are presented as mean ± SD; P values calculated by two‐tailed unpaired student’s t‐test.
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B–D
Infertility in Rb∆K7 males: (B) number of litters from breeding of homozygous Rb∆K7 (n = 4) or control wild‐type (n = 5) males with control wild‐type females. Bars represent mean ± SD; P values calculated by two‐tailed unpaired Student’s t‐test. (C) Representative images of testes from two Rb∆K7 mice compared with a control littermate. (D) Sections through seminiferous tubules. Arrows point to mature spermatids in control testes that are lacking in Rb∆K7 adult testes. Scale bar, 20 μm.
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E
Kyphosis in Rb∆K7 mice. Top, images of an eight‐month‐old Rb∆K7 mouse with kyphosis (right) and control wild‐type littermate (left). Bottom, radiographs of these mice under anesthesia showing abnormal cervical curvature (arrows) in the Rb∆K7 knock‐in mouse.
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F–I
Delayed hair re‐growth in eight‐month‐old Rb∆K7 mice two weeks post‐hair plucking (F). (G) Abnormal skin histology in Rb∆K7 mice relative to control. FCL, subcutaneous fat cell layer, T, telogen; A, anagen. (H and I) Representative immunostaining for keratin 14 and PCNA showing reduced expression in Rb∆K7 hair follicles vs. controls. Scale bar, 50 μm.
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J
Hyperglycemia in Rb∆K7 but not Rb∆K4 mice. Blood glucose in 10‐ to 12‐month‐old fasting Rb∆K7 (left, n = 11) or 18‐month‐old fasting Rb∆K4 (right, n = 15) mice versus control littermates (n = 6 and n = 8, respectively). Mean ± SD; P values by two‐tailed unpaired Student’s t‐test.
