Figure 7. Reduced TREM2 signaling does not rescue dysregulated lipids in Grn ^−/− mice.

• A–D
  Volcano plot presentation of lipids and metabolites upregulated (purple) or downregulated (blue) in total brain homogenates from 6‐month‐old male Grn ^−/− (A, n = 5), Trem2 ^−/− (B, n = 5), and Double ^−/− (C, n = 5) mice in comparison to WT (n = 4), and Double ^−/− in comparison to Grn ^−/− mice (D). Counts for each sample were normalized to the mean value of WT followed by a log2 transformation (n = 4–5 per genotype). Analyte values were adjusted with an FDR < 10% to exclude type I errors in null hypothesis testing.
• E–G
  Abundance of BMP species and glucosylsphingosine (GlcSph) in total brain of 6‐month‐old Grn ^−/−, Trem2 ^−/−, Double ^−/−, and WT mice (n = 4–5 per genotype).
• H
  Glucocerebrosidase (GCase) activity in whole‐brain lysates from 6‐month‐old male Grn ^−/−, Trem2 ^−/−, Double ^−/−, and WT mice. The linear increase in fluorescence signal was measured and then normalized to WT mice (n = 3–6 per genotype).

Data information: Data represent mean ± SEM. For statistical analysis, one‐way ANOVA with Tukey’s post hoc test of Grn ^−/−, Trem2 ^−/−, and Double ^−/− was used. Statistical significance was set at ***P < 0.001; ****P < 0.0001, and ns, not significant.

Source data are available online for this figure.