Figure 8.

Depletion of C1qa prevents Pg-induced synapse loss by microglia in vitro. (A) Representative Z-stack images of neuro-microglia (expressing shC1q or scramble vector) co-cultures in the presence or absence of Pg (MOI=50). Cultures were stained with IBA1 (red) and PSD95 (green). Scale bar: 5 µm. (B, C) Quantification of PSD95 puncta in the co-cultures (B) and in microglial cell bodies (C). (D) Western blot analysis of PSD95 puncta in FACS-sorted IBA1⁺ microglia lysates from the co-cultures. NeuN, neuronal marker. Densitometric analysis was performed using ImageJ software, and normalized to tubulin and expressed as fold changes over scramble control. Data are expressed as mean ± SEM of three independent experiments. *P < 0.05, ****P < 0.0001 by one-way ANOVA followed with Tukey correction.