FIGURE 3.

Characterization of reproductive 21‐nt phased secondary small interfering RNAs (phasiRNAs) in flax. (a) Expression of reproductive‐enriched multi‐microRNA (miRNA) triggered 21‐nt reproductive phasiRNAs in different tissue types. Left color‐coded panel indicates different trigger miRNA and their phasiRNA: green, miR390 triggered TAS3 loci; light blue, miR167 triggered TASL loci; dark blue, noncoding loci with unknown trigger, noncoding loci with miR482/2118 trigger; purple, miR167 derived metal protein‐related loci; and yellow, all other protein‐coding loci with unknown triggers. The key at right indicates the abundance in unit of log2(RP20M). B_2_4, B_5_7, and B_8_10 indicate bud stages 1, 2, and 3, respectively. (b) Upper track: Abundance (RP10M) of small RNAs (sRNAs) in both strands of an example TAS3 locus padded with 250 base pairs, each side. Name and coordinates of the PHAS locus are indicated in top. sRNA sizes are denoted by colors, as indicated at top. Below track: Phasing score of the same locus; the red dot indicates the highest phased sRNA position. (c) Similar to (b) for an example TASL locus as indicated in the top. (d) Alignment of members of miR167 in flax. The intensity of blue color denotes the conservation in nucleotide level. The consensus sequence of the alignment is shown with a sequence logo