Fig. 5.

Fluorescence images of the brain tissue slice of 20 month-old APP/PS1/TauP301L-BiFC mouse. (A) Staining protocol and the images; Step1: Sudan Black B stain, Step2: tau-BiFC fluorescence image acquisition. Step3; NAP-OB stain and fluorescence image acquisition, and Step4: image analysis. (B) Box and whisker plot analysis indicating the areas of amyloid plaques stained with NAP-OB. Out of 544 plaques detected in half brain tissue, 239 were tau-BiFC positive, and 305 plaques were tau-BiFC negative. (C) Magnified fluorescence images of the hippocampal subiculum. Yellow arrows indicate large plaques, which are co-stained with tau-BiFC and white circles indicate small plaques which are not stained with tau-BiFC. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)